Myricetin loaded nanoemulgel: in vitro characterization and anti- inflammatory efficacy assessment in Sprague Dawley rats

Abstract

Myricetin, a commonly found natural flavonoid, exhibit a wide range of potential therapeutic activity including antidiabetic, anticarcinogenic, neuroprotective, and antiinflammatory activity. It belongs to BCS class II and has poor aqueous solubility, which limits its therapeutic application in the treatment therapy. This study was aimed to design and characterize the nanoemulgel formulation of Myricetin for topical delivery with the possibility of improved solubility, skin penetration and antiinflammatory activities. In this work Myricetin loaded nanoemulgel was prepared by water titration method and evaluated for particle size, zeta potential, pH, spreadability, rheology, drug content, skin permeation, skin irritation studies. In-vivo anti-inflammatory study of optimized Myricetin nanoemulgel was compared with marketed preparation (Voveran® emulgel®). The prepared Myricetin nanoemulgel containing Carbopol 934P (2%) as a gelling agent, Tween 20 (20%) as a surfactant, Ethanol (20%) as a co-surfactant and Sefsol 218 (20%) as an oil. The optimized Myricetin nanoemulgel was highly stable and it showed no sign of edema, skin irritation. It also showed significant anti-inflammatory activity. Transdermal permeation study demonstrated higher penetration of Myricetin from nanoemulgel compared to the marketed emulgel (Voveran® emulgel®). The Myricetin nanoemulgel showed promising results and can be further considered as a potent natural alternative to the synthetic drug for the treatment of inflammation.

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