Research Article
Open access
Peer reviewed
CO2 Plasma Activated Water (PAW), a Novel Weapon for Hepatocellular Carcinoma
- Department of Chemical Sciences, College of Science and Education, University of Mkar, Mkar, P.M.B. 017, Benue State, Nigeria
- Department of Physical Sciences, College of Science and Education, University of Mkar, Mkar, P.M.B. 017, Benue State, Nigeria
- Received
- Published
DOI
https://doi.org/10.55006/biolsciences.2024.4209
Citation
Biological Sciences, Vol. 4 No. 2 (2024), 643-649
Abstract
Cancer poses significant challenges to global health, necessitating the exploration of innovative therapies. CO2 plasma activated water (PAW) holds promise as a novel therapeutic agent due to its selective cytotoxicity against cancer cells while sparing healthy tissues. This study investigated the in-vitro cytotoxic activity of CO2 PAW against human hepatocarcinoma (HepG2) cells. The cytotoxic effect of CO2 PAW on HepG2 cells was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cells were seeded in 96-well plates, treated with varying volumes of PAW. Morphological changes induced by PAW treatment were assessed using phase-contrast microscopy. The total RNA isolated from HepG2 cells, treated with PAW using the GF-1 total RNA extraction kit, qRT-PCR was performed using SYBR-green master mix with cDNA templates and synthesized primers. Data were analyzed using GraphPad Prism (ver. 9.5, USA, 1992) and presented as mean ± standard error of means. The results revealed dose-dependent cytotoxic effects, with higher concentrations inducing pronounced cell death. Morphological changes in HepG2 cells post-PAW treatment and gene expression analysis showed significant (P<0.05) alterations in Bax and Bcl-2 mRNA levels, indicative of intrinsic apoptotic pathway activation.
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