Research Article
Open access
Peer reviewed
Impact of Prolonged Use of Postinor®-2 and Norinyl®-1/35 on Hepatic Enzyme Levels in Female Wistar Rats: A Biochemical Assessment
- Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Benue State University, Makurdi, Benue State Nigeria
- Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, Baze University, Abuja, Nigeria
- Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Nigeria, Nsukka, Enugu State, Nigeria
- Received
- Published
DOI
https://doi.org/10.55006/biolsciences.2024.4203
How to Cite
Biological Sciences, Vol. 4 No. 2 (2024), 598-603
Abstract
The liver is prone to inflammation or disease after prolonged exposure to toxins or pharmaceutical agents. This study investigates the impact of prolonged exposure to contraceptive drugs, Postinor®-2 and Norinyl®-1/35, on hepatic enzyme levels in female Wistar rats. Twenty-five (25) adults female Wistar rats were divided into five groups (n = 5), respectively: Group 1 (control; administered 5 mg/kg/bw of normal saline). In groups 2 (Postinor®-2; treated with 0.10 mg/kg/bw), group 3 (Postinor®-2; 0.20 mg/kg/bw), groups 4 (Norinyl®-1/35; 0.10 mg/kg/bw) and group 5 (Norinyl®-1/35; 0.20 0.20mg/kg/bw) all admissions were done orally for fourteen (14) days. On day 15, the animals were anaesthetized, and their livers were harvested for biochemical analysis. After which, the results were analyzed using ANOVA with p<0.05 signifying the statistical significance. All the treated groups showed a significant increase in ALT levels compared to the control group; ALT was higher in group 2 (0.10 mg/kg b.wt of Postinor®-2) compared to group 3. While AST levels were reduced in groups 2 and 4 compared to the control group, SOD decreased in groups 1 and 4 when compared to the control group. MDA levels increase in contraceptive-administered groups compared with control group. In conclusion, prolonged intake of Postinor®-2 and Norinyl®-1/35, irrespective of dosage, induces oxidative stress by increasing lipid peroxidation, as observed by the value of MDA, and hence oxidative stress might be the reason by which the contraceptives alter the liver enzyme functions.
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